TABLE 1.
E. coli strains and plasmids used in this study (all K-12)
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Published strains | ||
| AB1157a | Wild type for DNA metabolism | 51 |
| KKW58a | ΔthyA72 ΔdeoCABD2 | 10 |
| ER15a | ΔseqA20::kan | 52 |
| KJK63a | ΔthyA72 ΔdeoCABD2 ΔrecBCD3::kan | 10 |
| KJK202a | ΔthyA72 ΔdeoCABD2 Δrep::kan | 10 |
| L392 | MG1655 dnaC2(Ts) thr::Tn10 | 53 |
| NS373 | dnaA46(Ts) tna::Tn10 | 54 |
| SRK253a | IOC Δkup::kan | 25 |
| CM2500b | dnaA601(Ts) | 55 |
| Strains from this study | ||
| KJK170a | ΔthyA72 ΔdeoCABD2 dnaA46(Ts) tna::Tn10 | KKW58 × P1 NS373 |
| RA101a | ΔthyA72 ΔdeoCABD2 dnaC2(Ts) thr::Tn10 | KKW58 × P1 L392 |
| SRK309-1a | dnaA46(T) tna::Tn10 | AB1157 × P1 NS373 |
| SRK270 | dnaA601(Ts) yidX+ cat yidA+ | Precise insertion in CM2500 |
| SRK270-1a | dnaA601(Ts) yidX+ cat yidA+ | AB1157 × P1 SRK270 |
| SRK271a | ΔthyA72 ΔdeoCABD2 ΔseqA20::kan | KKW58 × P1 ER15 |
| SRK290a | ΔthyA72 ΔdeoCABD2 IOC Δkup::kan | KKW58 × P1 SRK253 |
| SRK291a | ΔthyA72 ΔdeoCABD2 dnaA601(Ts) yidX+ cat yidA+ | KKW58 × P1 SRK270 |
| Plasmids | ||
| pEAK17-1 | 2.0-kb ter fragment in pCR TOPO2.1 | Elena Kouzminova, this lab |
| pEAK23-1 | 2.0-kb oriC fragment in pCR TOPO2.1 | Elena Kouzminova, this lab |
| pSRK-O | 2.0-kb oriC fragment of pEAK23-1 in pBluescript | |
| pSRK-OD | 2.0-kb oriC and ter fragments in pBluescript |
a
Other mutations: F– λ– rac thi-1 hisG4 Δ(gpt-proA)62 argE3 thr-1 leuB6 kdgK51 rfbD1 araC14 lacY1 galK2 xylA5 mtl-1 tsx-33 supE44(glnV44) rpsL31(strR).
b
Other mutations: λc+ metE46 trp-3 his-4 thi-1 galK2 lacY1 or lacZ4 mtl-1 ara-9 tsx-3 ton-1 rpsL8 or rpsL9 supE44.