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. 2019 Feb 15;18(4):1787–1795. doi: 10.1021/acs.jproteome.8b00981

Figure 2.

Figure 2

Comparison between retrieving cells from the LCM cap and digesting in situ in the LCM cap. As a pilot experiment, 100, 200, 400, and 800 Purkinje cells were collected using LCM and processed after centrifuging to collect the cells from the cap prior to digestion (A,B) or digested directly in the LCM cap (C,D). “By matching” refers to identifications matched from a sample of cerebellar cortex using MaxQuant’s “match between runs”. Digesting directly in the LCM cap results in an increase in peptide and protein identifications, especially at low cell numbers. (A) Number of peptides identified from Purkinje cells recovered from the LCM cap. (B) Number of proteins identified from Purkinje cells recovered from the LCM cap. (C) Number of peptides identified from Purkinje cells digested in the LCM cap. (D) Number of proteins identified from Purkinje cells digested in the LCM cap.