Figure 3.

Comparison of protein identifications of methods used for protein retrieval and digestion. To determine an optimal method of material collection, lysis and digestion, 60 000 μm³ of the molecular layer (the area of approximately 100 Purkinje cells) was collected to test different combinations of collection methods, lysis buffers and digestion methods. (A) Mean number of peptides identified in each combination. (B) Mean number of proteins identified in each combination. All bars are mean values (n = 3) and error bars represent standard deviation. In-Cap refers to the in situ digestion method. Cap and Buffer refer to collecting material onto both a dry LCM cap and recovering in the indicated lysis buffer, or collecting directly into the indicated lysis buffer. ISD: in-solution digestion.