HLA class Ι peptide
ligand identification. (A) Cumulative
unique peptides (black), predicted peptide ligands (green), and PSM
counts (gray) over repeated injections of the same unfractionated
sample. (B) Peptide identification overlap between repeated injections
of the same unfractionated sample. (C) Cumulative unique peptides
(black), predicted peptide ligands (red), and PSM (gray) counts over
all analyzed high-pH RP fractions. (D) Cumulative unique peptides
(black), predicted peptide ligands (blue), and PSM (gray) counts over
all analyzed SCX fractions. (E) Overlap between identified peptides
in high-pH RP (n = 10737, red) and SCX (n = 10511, blue). (F) Cumulative unique peptides (black), predicted
peptide ligands (orange), and PSM (gray) count over combined high-pH
RP and SCX fractions.