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. 2018 Jul 9;159(9):3132–3142. doi: 10.1210/en.2018-00433

Figure 4.

Figure 4.

α7nAChR siRNA regulates α7nAChR expression and GLP-1 secretion. (A and B) Cells were transfected with (+) or without (−) α7nAChR (α7-si) or nontargeting [scrambled (Scr)] siRNA for 48 h. Levels of α7nAChR (A) mRNA and (B) protein (α7-R) were measured by RT-PCR and Western blot analysis. Cells transfected with α7nAChR or Scr siRNA (48 h) were treated with GTS-21 (75μM) for 2 h, and GLP-1 secretion was determined. (C and D) Knockdown of α7nAChR reduced GLP-1 secretion induced by GTS-21 in (C) GLUTag and (D) STC-1 cells. ***P < 0.001 vs. scrambled siRNA group; ###P < 0.001 vs. scrambled siRNA plus GTS-21 group (n = 7 to 8).