Figure 1.

Peripartum fluoxetine exposure to C57BL/6 dams causes sustained reductions in femoral bone volume after weaning. Saline (n = 15) or 20 mg/kg fluoxetine (n = 14) was administered from the day of conception through day 21 of lactation. Mice were then aged to either 3 mo (n = 7, saline group; n = 7, fluoxetine group) or 9 mo (n = 8, saline group; n = 7, fluoxetine group) after weaning, at which time, the femurs were collected for microCT analysis. (A) Three-dimensional reconstruction images of distal femur trabecular bone. Scale bar, 100 µm. (B) BV/TV. (C) Blood samples were collected from all mice before any treatment (baseline), on the day of conception, and on days 1 and 21 of lactation, and serum was isolated. Serum calcium concentrations (mM) were generated by subtracting each mouse’s baseline serum calcium concentration from her serum calcium concentration on days 1 or 21 of lactation, respectively, to correct for variation within each dam. Data presented as mean ± SEM and analyzed using an SAS mixed linear model for treatment and time.