Figure 5.

Peripartum fluoxetine exposure did not affect the dam’s lactation. Saline (n = 4) or 20 mg/kg fluoxetine (n = 4) was administered from E13 through day 10 of lactation, at which time, the mice were euthanized. (A and B) The number four mammary gland was collected and histological hematoxylin and eosin staining was performed. Images were taken at ×20 magnification; scale bar, 15 µm. (C–E) The number of alveoli, diameter of alveoli, and number of adipocytes were quantified from three nonoverlapping sections for each mouse. (F and G) RNA samples were prepared from mammary gland tissues and subjected to quantitative RT-PCR for expression of α-lactalbumin and β-casein. Data presented as mean ± SEM and analyzed using a Student t test.