Table 1.
Primer Sequences Used for Quantitative Real-Time PCR
| Gene | Forward Primer 5′ → 3′ | Reverse Primer 3′ → 5′ |
|---|---|---|
| Alac | CTGCCTCTGAGCCTTGTACC | GTAAAACCCCCATCGAGACC |
| Bcas | GGTGAATCTCATGGGACAGC | GAGATGGTTTGAGCCTGAGC |
| Bglap | TTCTGCTCACTCTGCTGACC | ACCTTATTGCCCTCCTGCTT |
| Calbindin D9K | CCCGAAGAAATGAAGAGCATTTT | TTCTCCATCACCGTTCTTATCCA |
| Casr | TCTTGTGGAGTGGGTTCTCC | AAAACAGCAGGTGGGCTCT |
| Hprt1 | CTGGTGAAAAGGACCTCTCG | AACTTGCGCTCATCTTAGGC |
| Opg | AAGCTGGAACCCCAGAGC | GTGCTGCACTTCGTGTGTTT |
| Pmca1 | AACGACTGGAGCAAGGAGAA | CCGTACTTCACTTGGGCAAT |
| Pthrp | TTCCTGCTCAGCTACTCCGT | GATGGACTTGCCCTTGTCAT |
| Rankl | GGCCACAGCGCTTCTCAG | GAGTGACTTTATGGGAACCCC |
| Rps9 | GGAGACCCTTCGAGAAGTCG | GGGGATCCTTCTCGTCTAGC |
| Rps15 | TTGAGAAAGGCCAAAAAGGA | GTTGAAGGTCTTGCCGTTGT |
| Tph1 | TTCACCATGATTGAAGACAAC | TCCGACTTCATTCTCCAAGG |
Abbreviations: Alac, α-lactalbumin; Bcas, β-casein, Bglap, bone γ-carboxyglutamic acid–containing protein; Casr, calcium sensing receptor; Hprt1, hypoxanthine phosphoribosyltransferase 1; Opg, osteoprotegerin; Pmca1, plasma membrane Ca2+ ATPase 1; Pthrp, PTHrP, Rankl, receptor activator of nuclear factor κΒ ligand; Rps9, 40S ribosomal protein S9; Rps15, 40S ribosomal protein S15; Tph1, tryptophan hydroxylase 1.