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. 2018 May 10;159(7):2520–2527. doi: 10.1210/en.2018-00257

Figure 1.

Figure 1.

HDAC3 protein levels are lower in BAT than in iWAT and are reduced in both thermogenic fat depots after cold exposure. (A, B) Real-time qPCR analyses of (A) Hdac3 and Ucp1 (n = 3 per group) and (B) representative immunoblots of HDAC3, acetylated lysine, UCP1, and GAPDH as a loading control in BAT and iWAT isolated from C57BL/6J mice housed at room temperature. (C–F) Real-time qPCR analyses of Hdac3 and Ucp1 in (C) BAT (n = 3) and (E) iWAT (n = 3) and representative immunoblots of HDAC3, acetylated lysine, UCP1 and HSP90 as a loading control in (D) BAT and (F) iWAT isolated from C57BL/6J mice housed at room temperature or exposed to 10°C for 7 days. Data are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001. CE, cold exposure; RT, room temperature.