Fig. 7.

CDK5 shRNA silencing in L6 myotubes does not affect System A adaptation in response to AA insufficiency

L6 myotubes were stably transduced with lentiviral vectors expressing different shRNAs targeting murine CDK5. Cells were lysed, separated by SDS-PAGE and immunoblotted using antibodies targeting CDK5 and GPADH (A) or RNA isolated, cDNA synthesised and CDK5 mRNA assayed by quantitative PCR (B). Blots and bar graphs are representative of three individual experiments. Stably transduced L6 myotubes were amino acids deprived for 6 h in the presence/absence of 50 μM SB415286 or 30 μM roscovitine and MeAIB uptake assayed (C). Values are mean ± SEM (n = 3).