Figure 4.

Expression of CD68 and TNF-α in adipose tissue. Representative Western immunoblotting of (A) CD68 and (B) TNF-α proteins in OAT from NOW, OW, OBS, and GDM groups are shown. Relative densities of CD68 and TNF-α to β-actin were calculated and compared between groups. (C) The mRNA levels of TNF-α in adipose tissue were measured using RT-PCR and were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA expression. Data are displayed as the mean ± SEM. Different letters at the top of each bar indicate statistically significant differences between groups (P < 0.01).