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. 2019 Apr 10;16:77. doi: 10.1186/s12974-019-1465-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Myosin1f is required for neutrophil migration after acute ischemic stroke. A Myosin1f gene expression increased in blood neutrophils at 3 days after stroke compared to the sham control group(s), **** p < 0.0001, n = 3 mice/group; each has 3 replicates. B In vitro migration assay: the number of myosin1f ^+/+ neutrophils migrated from the upper chamber to the lower chamber was significantly higher compared to myosin1f^−/− neutrophils. *p = 0.0463, n = 3–4 mice/group; each has 3 replicates. C Confocal immunofluorescent staining for MPO, which was counterstained with DAPI. (a) A representative brain section stained with cresol/violet is presented to show the three indicated regions where cell numbers were quantified. (b) Representative staining of MPO⁺ (green) and DAPI (blue) at 72 h after stroke. (c) The bar graph represents the statistic results of the MPO⁺ positive cells. Scale bar: 50 μm. ** p = 0.0024, n = 5–6 mice/group. D FACS quantification of infiltrating neutrophils in the ischemic brain. (a) The representative gating strategies of CD45⁺, MiDMs (microglia-derived macrophages, CD45^LowCD11b⁺), MoDM (monocytes-derived macrophages, CD45^HiCD11b⁺), and neutrophils (CD11b⁺Ly6G⁺). (b) The cell numbers of total CD45⁺ leukocytes, neutrophils, MiDMs, and MoDMs. (*, **, ***, ****, p < 0.05, 0.01, 0.001, 0.0001, respectively. N = 3–5 mice/group.)