Figure 2.

Effect of dsRNA on the cell fusion activity of RSV. A549 monolayers were transfected with 20 nM of anti-P or anti-F dsRNA and infected with RSV as described under Materials and Methods. At 40 h p.i., the monolayers were examined under a Nikon TS100F phase-contrast microscope at 40× magnification and digitally photographed with a Nikon Coolpix 995 camera. Note the syncytia in 'A', cytopathic effect without syncytia in 'B', and monolayers that appear unaffected and identical in 'C' and 'D'.