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. 2019 Apr 1;10(2):231–248. doi: 10.14336/AD.2018.0510

Figure 1. Up-regulation of high-mobility group box 1 (HMGB1) in angiotensin (Ang) II-induced human aortic vascular smooth muscle cell (HAVSMC) phenotype transformation.

Figure 1.

A-D) mRNA levels of α-smooth muscle actin (α-SMA), smooth muscle 22α (SM22α), osteopontin (OPN), and HMGB1 in HAVSMCs, respectively. E) representative images of western blot analysis for HMGB1, α-SMA, SM22α, OPN, and GAPDH. F-I) Protein levels of α-SMA, SM22α, OPN, and HMGB1, respectively. Arbitrary optical density units of the target proteins were normalized to GAPDH and expressed as fold change. J. Protein levels of HMGB1 in culture medium *P <0.05, **P <0.01 vs. control group; #P <0.05, ##P <0.01 vs. Ang II group. At least three independent experiments were performed for each group.