Fig. 3.

ADP-dependent mitochondrial bioenergetics is altered following microtubule-targeted chemotherapy incubations in extensor digitorum longus permeabilized muscle fiber bundles. Complex I-supported (NADH from 5 mM pyruvate/4 mM malate) State 3 (+ADP) respiration was tested following incubations with (n = 6–8) paclitaxel (A) and vinblastine (B). Complex I-supported (10 mM pyruvate/4 mM malate) H₂O₂ emission in the absence (C and D) and presence of ADP (E and F) was determined following both paclitaxel and vinblastine incubations (n = 6–8). ADP-insensitive pyruvate dehydrogenase complex (PDC)-supported (10 mM pyruvate) H₂O₂ emission was assessed following paclitaxel (G) and vinblastine (H) incubations (n = 6–7). **P < 0.01 and ***P < 0.001 vs. control. Results are reported as means ± SEM.