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. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: J Immunol. 2019 Feb 22;202(8):2276–2286. doi: 10.4049/jimmunol.1800844

Figure 1.

Figure 1.

Shp1-deficient iNKT cells are biased towards iNKT2 and iNKT17 subsets. (A) Frequency (top row) and absolute numbers (bottom row) of iNKT cells in the indicated tissues of Shp1fl/fl and Shp1fl/fl CD4-cre mice. (B, C) iNKT cell subsets in the thymus (B) and spleen (C) of Shp1fl/fl (fl/fl) and Shp1fl/fl CD4-cre (cre) mice were assessed by PLZF and RORγt staining. (D) Frequency of iNKT cells in the thymus of single or mixed (mix) bone marrow chimeras. Rag1−/− mice were reconstituted with wild type CD45.1 and/or Shp1fl/fl CD4-cre CD45.2 bone marrow cells. (E, F) Frequency of iNKT2 and iNKT17 subsets in the thymus (E) and spleen (F) of 16-week-old Shp1fl/fl and Shp1fl/fl CD4-cre mice. Data shows representative dot plots, individual mice and the mean values +/− s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001, two-tailed unpaired Student t test.