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. 2019 Apr 3;10:269. doi: 10.3389/fphar.2019.00269

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Copyright © 2019 Qin, Rosli, Deo, Cao, Walsh, Tate, Alexander, Donner, Horlock, Li, Kiriazis, Lee, Bourke, Yang, Murphy, Du, Gao and Ritchie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ac_2-26 has no impact on myocardial neutrophil content 7 days post I-R injury in vivo. Representative immunofluorescent images of (A) LV neutrophil content (determined using anti-Ly6G⁺ antibody, and C) inflammatory monocytes (determined using anti-Ly6C⁺ antibody). Pooled data for both are shown in B) LV LyG⁺ and (D) LV Ly6C⁺ immunofluorescence. Images reveals overlay of dark blue (DAPI; detecting nuclei) and red (inflammatory cells) indicating positive staining (scale-bar = 50 μm). ^#P < 0.05 versus sham. One-way ANOVA with Tukey’s post hoc test. Data were presented as mean ± SEM, with number of mice per group. n = 7 (sham), n = 4 (I-R + vehicle), and n = 6 (I-R + Ac_2-26).