Fig. 1. Kinetics of photochemical deprotection of NB-caged DNA templates [DNA_UNB (A), DNA_CNB (B)] monitored by transcription (lanes 3–8). Lanes 1 and 2 show control transcriptions from natural DNA and DNA_Uhm or DNA_Chm, respectively. Representative primary data (DNA templates and RNA transcripts) are shown. The graphs in this and following Figures are averages of at 2–3 independent experiments ±SD. The time of irradiation and usage of two additives (DTT and NaN3) are indicated below the graphs.
