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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: J Bone Miner Res. 2018 Apr 25;33(7):1362–1375. doi: 10.1002/jbmr.3422

Figure:1.

Figure:1

(A) Detection of the deletion allele of Hdac4 by PCR in genomic DNA isolated from different tissues using primers arm1 and arm3. (B) Genotyping of Hdac4 mice using primers arm1 and arm2. Analysis of the expression of HDAC4 protein and Hdac4 mRNA in the heart. β-actin was used as a loading control. (C) Hdac4, and (D) Mmp13 levels relative to β-actin were determined in distal femur RNA from 10 week-old Hdac4fl/fl (n = 6) and Hdac4ob−/− mice (n = 8). Data are shown as fold changes compared with levels in Hdac4fl/fl mice. Data shown are means ± SE. *, p< 0.05 by Student’s t test.