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. Author manuscript; available in PMC: 2019 Apr 10.
Published in final edited form as: Cell Rep. 2019 Mar 19;26(12):3313–3322.e5. doi: 10.1016/j.celrep.2019.02.086

Figure 3. Codon-Level Ribosome Pause Score Analysis of Encoded Amino Acid Sequences across Fmr1-KO and Wild-Type Mice.

Figure 3.

(A–C) Log-log plots of mean pause scores calculated for (A) single amino acid, (B) di-, and (C) tri-amino acid sequences in Fmr1-KO and wild-type mice (n = 3 for both genotypes), with accompanying p value for the significance of the difference in these two distributions (Mann-Whitney U test). In each plot, the main diagonal is plotted as a blue line representing equal pausing in either genotype, highlighting the downward shift of the mass of individual sequences’ scores and decrease in pause score in Fmr1-KO mice.

(D–F) This shift is visualized differently in (D)–(F), histograms of the log-ratios of mean pause scores for every (D) mono- , (E) di-, and (F) tri-amino acid motif. The downward or rightward shift in (A)–(C) translates to a leftward shift away from the blue vertical line at x = 0, showing decreased pausing for the majority of encoded amino acid motifs in Fmr1-KO versus wild-type mice.