Figure 1.

Oxidized LDL and oxidized phospholipids induce phosphorylation of PLCγ2 in human and murine platelets.

(ai) Washed human platelets (5 × 10⁸ platelets/ml) incubated with apyrase, indomethacin, EGTA, and tirofiban were treated with nLDL (50 µg/ml) or oxLDL (50 µg/ml) for 2 min. Lysates were separated by SDS-PAGE and immunoblotted for pPLCγ2-tyr1217 (1:1000) and β-tubulin (1:1000). (aii) Quantification of three independent experiments, *p < 0.05. (bi) Human platelets treated with oxPC_CD36 (50 µM) or PAPC (50 µM) for 15 min analyzed as previously described. (bii) Quantification of three independent experiments, *p < 0.05. (ci) Murine platelets treated with oxPC_CD36 (25 µM) or PAPC (25 µM) for 15 min and analyzed as previously described. (cii) Quantification of three independent experiments, *p < 0.05. All immunoblots are representative of three independent experiments. (d) nLDL (3 µg) and oxLDL (3 µg) blotted for oxidized phospholipids by anti-oxPC antibody E06 (1:1000).