FIG. 5.

Perfusion system characterization. (A) Visualization of flow using LDPI shows perfusion occurring in the center of the agarose constructs while verifying that the impermeable silicone constructs obstructed the flow (arrowheads). Red color indicates greater perfusion. (B) At a pump rate of 0.24 mL/min, mixing between the two flow circuits was largely achieved within 24 h. At 0.01 mL/min, flow separation was maintained for ∼96 h (*p ≤ 0.05 compared with other groups, ^#p ≤ 0.1). LDPI, laser Doppler perfusion imaging. Color images are available online.