Figure 3.

Series of optical micrographs, which demonstrate the DEP trapping of single K562 cell (A), dispensing of single K562 cells into each chamber (B) and compartmentalization of single cells in each chamber with oil (C). To better show the results, enlarged view of figure A-C is provided in Supporting Information as Figure S16. (D) Fluorescence image of the entire device showing 96 reactions of single-cell LAMP amplification. Image is taken after 120 min of LAMP reaction. i) control experiment without K562 cells; ii) and iii) are two parallel tests for the analysis of BCR-ABL gene in single K562 cells in each chamber. (E) The fluorescence intensity profile along the randomly picked chambers from (i) and (ii) in panel D. (F) Optical micrograph of the chambers marked with red square in iii) of panel D before LAMP reaction. There was one empty chamber without a cell in iii), thus further confirming the fluorescence signal was generated by the single-cell LAMP reaction.