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. Author manuscript; available in PMC: 2019 Apr 10.
Published in final edited form as: ACS Appl Mater Interfaces. 2016 Nov 7;8(45):30735–30746. doi: 10.1021/acsami.6b10306

Figure 6.

Figure 6.

Effect of different amounts of added-PEI10K on in vitro gene transfection efficiency of HNPs. SUM159 cells were transfected with GFP expressing pDNA-loaded HNPs formulated with different PEG-P(asp)-Ad/PEI10K-CD/PEI10K ratios under a N/P ratio of 30/1. The pDNA transfections were performed at a dose of 3 μg of GFP expressing pDNA. Cells were incubated in the transfection solution for 48 h and then viewed and photographed under a fluorescent microscope. (a) The representative images (100×) of cells transfected with HNPs formulated with different amounts of PEI10K under a N/P ratio of 30/1. (b) The numbers of GFP expression cells per field of view (POV) counted under a fluorescent microscope. Results are from two independent experiments (n = 6), and three randomly selected fields were counted in each experiment. (c) The ζ-potentials of HNPs formulated with different amounts of PEI10K.