Table 5. Summary of features which have a significant association with sensitivity or resistance to autologous plasma or bN-mAb neutralization of EN1 viruses.

Feature in authentic sequences	Response to autologous plasma	EN1 plasma	Feature present in inferred sequences		Response to bN-mAb	bN-mAbs (P value)
			AN1	AN2		PG9	PG16	PGT121	PGT128	VRC01
+V2 insertion	Resistant	<0.0001	-	+	Resistant	0.0006	0.0043	0.0001	0.3132	0.1512
+N444	Resistant	0.0035	-	-	Resistant	0.0287	0.0503	0.0298	0.1129	0.0944
+N402	Sensitive	0.0017	-	-	Sensitive	0.0025	0.0104	0.0087	0.4974	0.3094
+N130	Resistant	0.0290	-	+	Resistant	0.0068	<0.0001	0.1338	0.5622	0.9718
+N339	Sensitive	0.0007	-	-	Sensitive	0.0375	0.0010	0.1079	0.9447	>0.9999
+N337	Resistant	0.0290	+	+	Resistant	0.2284	0.0196	0.5572	0.6360	0.5945
+N137	None	0.0720	-	+	Sensitive	0.0690	0.1554	0.0172	0.1180	0.6011
+N295	Sensitive	0.0392	-	-	None	0.4160	0.1041	0.6617	0.4665	0.4215
+N413	Sensitive	0.0353	+	+	None	0.6057	0.1291	0.4308	0.1180	0.9169

PNGS features have HXB2 numbering. The associated response (sensitive, resistant or none) to each feature by autologous plasma or bN-mAbs was calculated by comparing the mean neutralization titers of viruses exhibiting the sequence feature to viruses that did not have the sequence feature (Mann-Whitney U-test (p = <0.05). Statistically significant responses are indicated by P values in bold.