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. 2019 Apr 10;15(4):e1008068. doi: 10.1371/journal.pgen.1008068

Fig 3. Overexpression of REF6 and NYE1 in each other’s mutants confirms their roles in promoting the general leaf senescence process.

Fig 3

(A) Leaf phenotypes of ref6-1+PiDEX::NYE1 transgenic plants treated with or without DEX on 3 DAD. -1, -3, and -4 represent different transgenic lines. (B, C) Chl contents (B) and Fv/Fm ratios (C) in the leaves shown in (A). (D) Leaf phenotypes of nye1-1+PiDEX::REF6 transgenic plants treated with or without DEX on 3 DAD. -1, -2, and -5 represent different transgenic lines. (E, F) Chl contents (E) and Fv/Fm ratios (F) in the leaves shown in (D). (G-I) H2O2 levels detected by DAB staining (G), Fv/Fm ratios (H), and Ion leakage (I), in ref6-1, Col-0, and ref6-1+PREF6::REF6-HA plants on 4 DAD. Data are mean ± SD (n = 10). *P < 0.05, **P < 0.01, ***P < 0.001 by unpaired Student’s t test.