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. 2019 Apr 10;14(4):e0215340. doi: 10.1371/journal.pone.0215340

Fig 1. ChIP-seq comparison between Santa Cruz (sc-543), Millipore (06–935) and Abcam (ab3575) antibodies.

Fig 1

A) Venn diagram showing the overlap between ERα binding sites for Santa Cruz (sc-543), Millipore (06–935) and Abcam (ab3575) antibodies in MCF7 cells. B) Pearson's correlation between each replicate of all three antibodies in MCF7 cells. C) Top: De novo motif analysis of ERα binding sites using MEME. Bottom: Heatmap of total number of ERα binding sites identified in both technical replicates of MCF7, and in all three biological replicates for MDA-MB-231, respectively. D) Examples of ERα- bound regions. Tag densities are shown as reads per million.