Fig 4. Cone outer segment degeneration progresses with age in cep290^fh297/fh297 mutants.

Immunohistochemistry of retinal cryosections stained with peanut agglutinin (PNA) to label cone outer segments and Zpr-1 (green) to label red/green double cones of wild-type and cep290^fh297/fh297 mutants. Views from dorsal retinas are shown. (A-F) Retinas from 3-month old adults. (G-L) Retinas from 6-month old adults. (M-R) Retinas from 12-month old adults. Arrows denote cones that were negative for PNA but positive for Zpr-1. ROS, rod outer segments; COS, cone outer segments; CIS, cone inner segments; ONL, outer nuclear layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bar: 50 μm.