Fig 6. Mislocalization of rhodopsin in cep290^fh297/fh297 mutants.

(A-D) Images show cryosections labeled with Zpr3 (red) to mark rhodopsin and DAPI (blue) to label nuclei in the dorsal retinas from cep290^fh297/fh297 mutants and wild-type siblings at 3 months of age; (E-H) 6 months of age, and (I-L) 12 months of age. At later ages, the distance between the base of the rod outer segments and the outer nuclear layer decreases due to loss of cone nuclei (I, J; white brackets). Arrowheads note rhodopsin mislocalization. ROS, rod outer segments; COS, cone outer segments; CIS, cone inner segments; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer. Scale bar: 100 μm.