Fig 8. PCNA localization in cep290^fh297/fh297 mutants at 3 and 6 months of age.

(A) PCNA immunolocalization in cryosections of the dorsal retina of wild-type (top) and cep290^fh297/fh297 mutants (bottom) at 3-months and 6-months of age. (B) PCNA positive cells were quantified in the INL from cryosections of the both dorsal and ventral retina at different ages. (C) Quantification of PCNA in the ONL from cryosections across the dorsal and ventral retina at different ages. A significant increase in PCNA immunoreactivity was seen in both the INL and ONL of cep290^fh297/fh297 mutants at 6 months of age. Quantification was performed on cryosections of individual retinas from cep290^fh297/fh297 mutants (n = 6) and wild-type siblings (n = 5) at the stated ages. Values represent the mean ± s.e.m. *P < 0.05; **** P < 0.0001 as determined by an unpaired t-test. ONL, outer nuclear layer; INL, inner nuclear layer; RGC, retinal ganglion cells. Scale bar: 50 μm.