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. Author manuscript; available in PMC: 2020 Mar 4.
Published in final edited form as: Curr Biol. 2019 Feb 21;29(5):823–833.e5. doi: 10.1016/j.cub.2019.01.056

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and Virus Strains
RNAi feeding strain Caenorhabditis Genetics Center (CGC) HT115(DE3)
Ahringer RNAi Library Source Bioscience C. elegans RNAi Collection (Ahringer)
bacterial food: E. coli OP50 strain CGC OP50
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
hygromycin B from Streptomyces hygroscopicus Millipore Sigma CAS 31282-04-9
2,3-Butanedione monoxime (BDM) anesthetic Millipore Sigma CAS 57-71-6
Critical Commercial Assays
Q5 site-directed mutagenesis kit New England Biolabs Catalog #E0554S
Experimental Models: Organisms/Strains
C. elegans unc-119(ed4) for transgene rescue Paul Sternberg PS3460
axIs1677(pie-1p::GFP::H2B ::pgl-1 3′utr + unc-119(+)); qyIs46(emb-9p::emb-9::mCherry) X WB Cat# JH2320, RRID:WB-STRAIN:JH2320 from CGC NK2386
qyIs86(egl-13p::GFP); naSi2(mex-5p::H2B::mCherry::nos-2 3′UTR) II NK574 parent from [62] NK1671
qyIs448(myo-3p::mCherry::PLCδPH); xnSi1(mex-5p::GFP::PLCδPH::nos-2 3′UTR) II FT598 parent from [63] NK1865
qyIs473(myo-3p::GFP::CAAX); gld-2(q497) gld-1(q485) /hT2 I; naSi2(mex-5p::H2B::mCherry::nos-2 3′UTR) II WB Cat# JK2879, RRID:WB-STRAIN:JK2879 parent from CGC, [23] NK2515
zcIs18(ges-1p::GFP); naSi2(mex-5p::H2B::mCherry::nos-2 3′UTR) II WB Cat# SJ4144, RRID:WB-STRAIN:SJ4144 parent from CGC NK1655
naSi2(mex-5p::H2B::mCherry::nos-2 3′UTR) II; mgIs49(mlt-10p::GFP-PEST) IV WB Cat# GR1438, RRID:WB-STRAIN:GR1438 from [64] NK2516
qyIs287[myo-3p::mCherry]; oxIs363(unc-122p::GFP) WB Cat# EG5071, RRID:WB-STRAIN:EG5071 parent from CGC [56] NK1676
qyIs287[myo-3p::mCherry]; qyIs245[zmp-5p::GFP] NK1803 parent from Kelley et al., in press NK1690
smIs119(hsp::ttr-52::mCherry) [25] transgene smIs119
qyIs473(myo-3p::GFP::CAAX); ced-1(e1735) I; naSi2(mex-5p::H2B::mCherry::nos-2 3′UTR) II WB Cat# CB3203, RRID:WB-STRAIN:CB3203 parent from CGC NK2185
qyIs473(myo-3p::GFP::CAAX); unc-40(e271) I; naSi2(mex-5p::H2B::mCherry::nos-2 3′UTR) II WB Cat# CB271, RRID:WB-STRAIN:CB271 parent from CGC NK2037
qyIs353(lag-2p::GFP::CAAX); naSi2(mex-5p::H2B::mCherry::nos-2 3′UTR) II nasi2 transgene from [65] NK1770
qIs154(lag-2p:: myr::tdTomato); lag-2(cp193[lag-2::mNeonGreen^3xFlag])V WB Cat# JK4472, RRID:WB-STRAIN:JK4472 parent from CGC, [13] NK2517
qyIs287[myo-3p::mCherry]; lag-2(cp193[lag-2::mNeonGreen^3xFlag]) V This study NK2376
cpIs121(lag-2p::mNG:: PLCδPH::F2A::rde-1) I; rrf-3(pk1426) II; rde-1(ne219) V [14] LP718
cpIs121(lag-2p::mNG:: PLCδPH::F2A::rde-1) I; rrf-3(pk1426) II; sax-7(eq1) IV; rde-1(ne219) V WB Cat# LH81, RRID:WB-STRAIN:LH81 parent from CGC, [66] NK2035
hmr-1(cp21[hmr-1::GFP+LoxP]) I; cpIs91(lag-2p::2x mKate2::PH::3xHA::tbb-2 3′UTR LoxN) II WB Cat# LP172, RRID:WB-STRAIN:LP172 parent from [41] NK2518
cpIs91(lag-2p::2x mKate2::PH::3xHA::tbb-2 3′UTR LoxN) II; hmp-1(cp20[hmp-1::GFP + LoxP unc-119(+) LoxP]) V WB Cat# LP191, RRID:WB-STRAIN:LP19 parent from [41] NK2364
hmp-2(cp78[GFP::hmp-2A]) I; cpIs91(lag-2p::2x mKate2::PH::3xHA::tbb-2 3′UTR LoxN) II WB Cat# LP316, RRID:WB-STRAIN:LP316 parent from [41] NK2368
ddIs290[sax-7::TY1::EGFP::3xFLAG(92C12) + unc-119(+)] II; cpIs91[lag-2p>2x mKate2::PH::3xHA::tbb-2 3′UTR LoxN] II (heterozygotes) WB Cat# TH502, RRID:WB-STRAIN:TH502 parent from CGC NK2451
qyIs450(myo-3p::mCherry:: PLCδPH); hmr-1(cp21[hmr-1::GFP+LoxP]) I WB Cat# LP172, RRID:WB-STRAIN:LP172 parent from [41] NK2173
qyIs455(myo-3p::mCherry:: PLCδPH); hmp-1(cp20[hmp-1::GFP + LoxP unc-119(+) LoxP]) V WB Cat# LP191, RRID:WB-STRAIN:LP19 parent from [41] NK2371
qyIs455(myo-3p::mCherry:: PLCδPH); hmp-2(cp78[GFP::hmp-2A]) I WB Cat# LP316, RRID:WB-STRAIN:LP316 parent from [41] NK2373
qyIs450(myo-3p::mCherry:: PLCδPH); ddIs290[sax-7::TY1::EGFP::3xFLAG(92C12) + unc-119(+)] II WB Cat# TH502, RRID:WB-STRAIN:TH502 parent from CGC NK2439
Oligonucleotides
myo-3 promoter F 5’-TTCCCGACAAAACATGAG-3′ This study N/A
myo-3 promoter R 5’-AGATGGATCTAGTGGTCG-3′ This study N/A
lag-2(cp193) 5’ homology arm F
5’-CTTGCAGTGTCAAAGACGCC-3′		 This study N/A
lag-2(cp193) 5’ homology arm R
5’-CTGGAATAGACGGTGGACTTAA-3′		 This study N/A
lag-2(cp193) 3′ homology arm F
5’-TAGATTCTTCCTTTATCCAAAAAATGTGT-3′		 This study N/A
lag-2(cp193) 3′ homology arm R
5’-CAATTTGGCGGCGGGATAGA-3′		 This study N/A
lag-2(cp193) guide RNA primer
5’-GAAGAATCTAGACATAGTGAC-3′		 This study N/A
hmr-1 RNAi F 5’-ATGCAGAAGCGGCGGTGCACGT-3′ This study N/A
hmr-1 RNAi R 5’-TGAACGGAGAACACATCAAC-3′ This study N/A
Recombinant DNA
epi-1 feeding RNAi clone Ahringer RNAi library [57] HGMP_Location
IV-5J23
dgn-1 feeding RNAi clone Ahringer RNAi library [57] HGMP_Location
X-5C20
hmr-1 feeding RNAi clone Ahringer RNAi library [57] HGMP_Location
I-5F19
Improved hmr-1 feeding RNAi clone This study pLML30
Membrane expression vector with mCherry:: PLCδPH [67] pAA173, Addgene plasmid #21743
Membrane expression vector with GFP::CAAX Scott Alper pSA129
Plasmid for SEC CRISPR strategy [33] pDD268
eft-3p::Cas9+sgRNA expression vector [33] pDD162, Addgene plasmid #47549
RNAi empty vector for new RNAi cloning and controls Andrew Fire L4440, Addgene plasmid #1654
myo-3p::mCherry [56] pCFJ104
unc-119 rescue plasmid Morris Maduro pPD#MM016B
Software and Algorithms
μManager software v1.4.18 [60] https://micro-manager.org/
Axiovision software Carl Zeiss https://www.zeiss.com/microscopy/us/products/microscope-software/axiovision.html
FIJI 2.0 [68] https://fiji.sc/
Photoshop CC Adobe Systems Inc. N/A
Adobe Illustrator CC Adobe Systems Inc. N/A
FIJI stitching plug-in [61] https://imagej.net/Image_Stitching
Pic Stitch – Collage Editor Big Blue Clip, LLC https://itunes.apple.com/us/app/pic-stitch-collage-editor/id454768104?mt=8
JMP Pro 13 JMP®, Version 13. SAS Institute Inc., Cary, NC, 1989–2007.
 https://www.jmp.com/en_us/software/predictive-analytics-software.html
Imaris Bitplane, Inc. Versions 7 and 9