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. 2019 Feb 28;12(1):51–62. doi: 10.15283/ijsc18061

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Copyright © 2019 by the Korean Society for Stem Cell Research

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — Organotypic multicellular spheroid (OMS) formation after 5 days: time vs. cell density. (A~C) Relation 1:5:5 MSC:Ec:HSC (×20, inverted microscopy. Scale bar: 5 mm), (D~F) Relation 1:2:2 MSC:Ec:HSC (Total cell density 1×10⁵ cells) (×20, inverted microscopy. Scale bar: 5 mm). (G) Morphology of OMS after 5 days of culture. It shows cells that are organized in the periphery of the structure demilitarizing the sphere (Relation 1:2:2 MSC:Ec:HSC. ×40, hematoxylin & eosin. Scale bar: 5 mm). (H) DNA quantification in the spheres of the two experimental conditions (p<0.05).