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Gene expression analysis of TGFβR1, TGFβR2 and Sox9 normalized to HPRT in MSC pellet cultures under chondrogenic and hypertrophy enhancing conditions analyzed by real time PCR. TGFβR1 is significantly down-regulated under hypertrophic conditions on day 17, 21 and 28 (A). TGFβR2 is down-regulated under hypertrophic conditions on day 28 (B). Sox9 is down-regulated on day 17 and 21 in hypertrophic MSC pellets (C). n=7 different donors.
