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Primer sequences for real time PCR
| Gene | Sequence (forward) | Sequence (reverse) | Concen-tration |
|---|---|---|---|
| HPRT | CGAGATGTGATGAAGGAGATGG | GCAGGTCAGCAAAGAATTTATAGC | 150 nM |
| Sox9 | ACACACAGCTCACTCGACCTTG | AGGGAATTCTGGTTGGTCCTCT | 200 nM |
| TGFβR1 | GAACCTGCTCTCCTGCTTG | GCCTCATCTGCTCAATCTCC | 200 nM |
| TGFβR2 | GGAAACTTGACTGCACCGTT | CTGCACATCGTCCTGTGG | 200 nM |
| TGFβ1 | ACATCAACGGGTTCACTAC | CTGAAGCAATAGTTGGTGTCC | 200 nM |
| TGFβ3 | ACACACAAGCAACAAACCTCAC | AACCAAACCCACACTTTCTTTACC | 200 nM |
