Fig. 1.

Schematic representation of the culture system used for hematopoietic cell differentiation from mouse ES cells. For hematopoietic EB formation, ES cells were differentiated with the methylcellulose medium with SCF for 10 days. For secondary differentiation, EBs were harvested and disrupted into single cells and replated with cytokines (SCF, IL-3, IL-6, and EPO) in the presence or absence of SIRT1 inhibitors. Counting of the colony numbers, RT-PCR and FACS analyses were performed at the indicated time points.