Fig. 2.
SIRT1 inhibitors enhance hematopoietic colony forming ability during hematopoietic differentiation of mES cells. (A) Colony-forming potential of mES-derived cells in the presence of nicotinamide 0.5 mM (NAM) and splitomicin 60 μM (SP). ES cells were differentiated by EB formation for 10 days. EBs were disrupted into single cells and re-plated in 1% methylcellulose medium containing a cocktail of hematopoietic cytokines. Phase-contrast images were taken from representative BFU-E, CFU-E, CFU-GM and CFU-GEMM colonies after 7 days. Scale bar. 100 μm. (B) After the colony-forming assays, the numbers of BFU, CFU-E, CFU-GM, CFU-GEMM, and 2nd EB were scored by microscopy and compared. Each bar is expressed as the mean±standard error of the mean. *p<0.05, **p<0.01, ***p<0.001 (vs. control) of n=9 independent experiments. (C) The results are shown in percentage terms. Percentages represent the proportion of each colony type.