Figure 2. Movement and reward related signals in ChI populations differ across the dorsal-ventral axis of the striatum.

(A) Representative population fluorescence changes (DF/F, green) and velocity (black) from dorsal (top) and ventral (bottom) striatum ChIs during spontaneous movement. Red indicates identified significant positive-going transients. (B) Mean population DF/F (top) and acceleration (bottom) aligned on movement initiations from rest across all dorsal (n = 118 onsets, three mice) and ventral (n = 66 onsets, three mice) ChI recordings without reward deliveries. (C) Mean acceleration (top) and transient population DF/F (bottom) aligned on onsets of significant positive-going transients across dorsal (n = 1215 transients) and ventral (n = 851) ChI recordings without reward. (D) Mean DF/F and spout licking aligned on the unpredicted triggering of the solenoid reward valve for dorsal (n = 127 rewards, 10 mice) and ventral (n = 112 rewards, eight mice) ChI population recordings. (E) DF/F for all unpredicted reward deliveries aligned on solenoid valve trigger for dorsal ChI recordings sorted by peak response. (F) Mean dorsal ChI DF/F for reward deliveries where the mouse stopped to consume the reward from locomotion (bottom quartile of velocity changes at reward delivery, n = 32) or accelerated from a resting state (top quartile, n = 32). (G) Mean velocity (top) and acceleration (bottom) for the deliveries plotted in F. (H) Change in velocity vs the mean dorsal ChI DF/F for each reward delivery. (I) Same as E for ventral ChI recordings. (J) Same as F for ventral ChI recordings (n = 28). (K) Same as G for ventral recordings. (L) Same as H for ventral recordings. Shaded regions represent ±SEM.

Figure 2—figure supplement 1. Dorsal and ventral ChI populations respond differently to unpredicted reward consumption and to conditioned cues.

(A) Mean DF/F from ventral striatum ChIs aligned on movement onsets from rest before (n = 78, three mice) and after (n = 253, eight mice) exposure to unpredicted rewards. (B) Mean DF/F (top), spout licking (mid), and velocity (bottom) from dorsal and ventral ChIs aligned on the first spout lick following water reward delivery (same deliveries as Figure 2D). Shaded red region represents the period where the mean ventral striatum DF/F was significantly (p<0.01, Wilcoxon Rank-Sum Test) elevated relative to pre-reward baseline. (C) Dorsal striatum ChI population DF/F for all classical conditioning trials in a single session (top) and the mean DF/F across these trials (bottom). Blue line above plot indicates the time when the CS (blue light) was on. (D) Corresponding velocity traces for all of the trials in C. (E) Ventral striatum ChI population DF/F for all classical conditioning trials in a single session (top) and the mean DF/F across these trials (bottom) from the same mouse as C. (F) Corresponding velocity traces for all of the trials in E. (G) Mean ChI population DF/F (top), velocity (mid), and spout licking (bottom) across all classical conditioning trials from dorsal (n = 73, four mice) and ventral (n = 71, three mice) recordings. Shaded regions represent ±SEM.