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. 2019 Mar 28;8:e44903. doi: 10.7554/eLife.44903

Figure 3. Single ChIs signals are rapidly elevated at movement onsets and throughout locomotion.

(A) Mean fluorescence projection image from 2-photon imaging of a representative field containing multiple distinct GCaMP6f expressing ChIs. (B) DF/F for each of the ROIs in A during a representative behavior period (velocity, black). (C) Mean fluorescence projection image of a single dorsal striatum ChI. Red ROIs indicate dendritic branches. (D) DF/F from the ROIs in C. Note the presence of synchronized transients across all dendrites (period in dashed box zoomed at bottom). (E) Mean transient DF/F during rest and movement periods for all ChIs somata (n = 268 neurons, six mice). (F) Mean transient DF/F across ChIs somata and dendrites for rest, movement onset, and continuous locomotion periods. (G) Mean DF/F (top) across all ChIs and velocity (bottom) aligned on clean movement onsets from rest. (H) Peak normalized mean DF/F for each ChI soma (top) and velocity (bottom) sorted by peak responses at movement initiation. (I) Mean DF/F (top) across ChI somata (n = 96 cells, six mice) and velocity (bottom) aligned on clean locomotion initiations from rest. (J) Mean DF/F (top) across ChI somata (n = 213 cells, six mice) and velocity (bottom) aligned on clean jerk onsets from rest. *p<0.01, **p<1e-8, Wilcoxon rank sum test. Shaded regions represent ±SEM.

Figure 3.

Figure 3—figure supplement 1. Movement related ChI signals are not generated by motion artifact.

Figure 3—figure supplement 1.

(A) Mean fluorescence projection image of a single dorsal striatum ChI expressing Td-tomato. (B) Velocity (top) and DF/F (bottom) from the td-tomato expressing ChI in A. Note the absence of significant positive-going transients relative to the noise. (C) Mean DF/F across all Td-tomato expressing ChIs (n = 18) aligned on movement onsets from rest. (D) Mean transient DF/F during rest and movement periods for all ChIs expressing Td-tomato (n = 18 neurons, two mice). (E) Mean fluorescence projection image of dorsal striatum ChIs expressing GCaMP6f during 800 nm excitation. (F) DF/F and velocity during a representative period for each of the ChIs indicated in (E) during 920 nm excitation. (G) DF/F and velocity during a representative period during 800 nm excitation for each of the ChIs indicated in E. Note the absence of significant positive-going transients in the absence of calcium sensitivity with 800 nm excitation, despite similar static baseline fluorescence (E). Shaded regions represent ±SEM.