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. Author manuscript; available in PMC: 2019 Dec 18.
Published in final edited form as: Chembiochem. 2018 Nov 15;19(24):2550–2552. doi: 10.1002/cbic.201800599

Figure 2.

Figure 2.

A. A series of triazabutadienes was synthesized and evaluated for their ability to label proteins. B., C. and D. are scans from SDS-PAGE gels. Fluor. = fluorescence scan; Coom. = coomassie blue staining. B. Minimal difference was observed when bovine serum albumin (BSA) was treated with compounds 2, 5-7 at pH 5 for 2 hours. The samples were quenched with resorcinol and then run on a gel and analyzed. C. BSA was incubated with 2, 5-7 in pH 7 PBS buffer and UV irradiation for either 0 or 15 minutes by incubation for an additional 45 minutes in the absence of light. The samples were then run on a gel and analyzed. D. A sample of E. coli lysate was incubated with 6 in Tris-HCl buffer pH 8.8 for 40 minutes in the presence or absence of UV light followed by incubation for an additional 2 hours in the absence of light. The samples were then run on a gel and analyzed.