Skip to main content
. Author manuscript; available in PMC: 2019 Apr 10.
Published in final edited form as: Nature. 2017 Nov 8;551(7680):389–393. doi: 10.1038/nature24484

Figure 1. The ASCC complex forms foci upon alkylation damage.

Figure 1.

(a) Images of ASCC3 and pH2A.X immunofluorescence after treatment with damaging agents. (b) ASCC3 foci quantitation (n=3 biological replicates; mean ± S.D.; two-tailed t-test, * = p < 0.001). (c) PLA images in control or MMS-treated cells using 1meA and ASCC3 antibodies (n=3 biological replicates). (d) Immunofluorescence of HA-ASCC2 expressing cells treated with MMS. (e) Quantitation of MMS-induced co-localizations of HA-ASCC2 foci (n=3 biological replicates; mean ± S.D.). Scale bars, 10 μm.