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. Author manuscript; available in PMC: 2019 Apr 10.
Published in final edited form as: Nature. 2017 Nov 8;551(7680):389–393. doi: 10.1038/nature24484

Extended Data Figure 7. ASCC2 coordinates ASCC-ALKBH3 complex recruitment during alkylation damage.

Extended Data Figure 7.

(a) Whole cell lysates from Extended Data Figure 6i (left) Figure 3e (right) and (right) were collected and expression was analyzed by Western blotting (n=2 independent experiments). (b) Immunoprecipitation of HA-ASCC2 or HA-ASCC2 L506A was performed and analyzed by Western blot as shown (n=2 biological replicates). (c) Flag-ASCC2 or Flag-ALKBH3 were immobilized and tested for binding to full-length (FL) His-ASCC3. (d) Flag-ASCC2 or Flag-ALKBH3 were immobilized and tested for binding to N-terminally deleted His-ASCC3 (His-ASCC3-ΔN) (n=2 independent experiments). (e) Flag-ALKBH3 was immobilized and tested for binding to His-ASCC2, with His-ASCC3-C (C-terminus of ASCC3) serving as a positive control (n=2 independent experiments). (f) ASCC-ALKBH3 complex model.