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. Author manuscript; available in PMC: 2019 Apr 10.
Published in final edited form as: Nature. 2017 Nov 8;551(7680):389–393. doi: 10.1038/nature24484

Figure 4. RNF113A ubiquitination recruits the ASCC complex.

Figure 4.

(a) MMS-induced foci in U2OS cells expressing indicated shRNAs (n=3 technical replicates; mean). (b) Ubiquitin ligase assays using E1, E2 (UBC13/MMS2; 250 nM), and Flag-RNF113A. K63R ubiquitin was substituted as shown (n=2 independent experiments). (c) Images of X-TTD or control lymphoblasts expressing the indicated vectors after MMS (n=3 technical replicates; mean). (d). ASCC2 interactome analysis. UBC13 substrates were previously described19. (e) HA-RNF113A deletions were immunoprecipitated to analyze BRR2 interaction. (n=3 independent experiments). (f) Images of U2OS cells expressing indicated shRNAs. Numbers indicate the percentage of cells expressing at least five (c) or ten (a) foci. Scale bars, 10 μm.