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. Author manuscript; available in PMC: 2019 Apr 10.
Published in final edited form as: Nature. 2017 Nov 8;551(7680):389–393. doi: 10.1038/nature24484

Extended Data Figure 5. ASCC2 binds specifically to K63-linked ubiquitin chains.

Extended Data Figure 5.

(a) and (b) His-ASCC2 or the indicated His-ASCC2 deletions were immobilized on Ni-NTA and assessed for binding to K63-Ub2–7 (a) or K48-Ub2–7 (b) (n=3 independent experiments). (c) Schematic of ASCC2 or different ASCC2 deletions and their observed respective binding towards K63-Ub2–7 or K48-Ub2–7. N.D., not determined. (d) Sequence alignment and conservation of residues 373–415 of human ASCC2. (e) Interaction model between ubiquitin and the CUE domain of ASCC2 (PDB ID: 2DI0). The positions of four residues (L478, L479, P498, and L506) are shown. (f) Binding assays were performed with K63-Ub2–7 using WT or the mutants of His-ASCC2 (n=3 independent experiments).