Table 1:
Strains and Plasmids used in this study.
| Strains or plasmids | Genotype/description | Source/Reference |
|---|---|---|
| E.coli | ||
| DH5α | E. coli DH5α for cloning | Novagen |
| BL21 | E. coli for protein expression | Novagen |
| ALC8856 | E. coli BL21 with pET14a-6x-His-YpdA | This study |
| ALC8857 | E. coli BL21 with pET14a-6x-His-YpdA-G10A | This study |
| S. aureus | ||
| RN4220 | Restriction minus NCTC8325-4 | (Kreiswirth et al., 1983) |
| ALC7446 | SH1000 corrected for BSH production | (Posada et al., 2014) |
| ALC8254 | ΔypdA::kan, ALC7446 with ypdA replaced by kanamycin cassette | This study |
| ALC8638 | ΔypdA::Δkan::ypdA, ALC8254 chromosomally complemented with ypdA | This study |
| ALC8858 | ALC7446 containing pALC8858 (promoter of ypdA driving venus) | This study |
| ALC8859 | ALC7446 containing pALC7925 (empty vector) | This study |
| ALC8860 | ALC7446 containing pALC7926 | This study |
| Plasmids | ||
| pET14b | E. coli expression vector | Novagen |
| pMAD | E. coli/S. aureus shuttle plasmid with the ori pE194ts; bgaB Ampr Ermr | (Arnaud et al., 2004) |
| pSK236 | Shuttle vector containing pUC19 cloned into the HindIII site of pC194 | (Gaskill and Khan, 1988) |
| pALC7925 | pALC2073 derivative with improved −10 binding site as described by Corrigan and Foster | This study, (Bateman et al., 2001; Corrigan and Foster, 2009) |
| pALC7926 | pALC7925 with ypdA for overexpression (pALC7925::ypdA) | This study |
| pALC8858 | pSK236 with promoter of ypdA driving venus | This study |