Table 1.
List of primary antibodies, their source and working dilutions. Abbreviations: IHC, immunohistochemistry; IF, immunofluorescence.
Primary antibody | Host species | Catalog no. | Producer | Dilution |
---|---|---|---|---|
anti-CD34 | Mouse | M7165 | Dako, Glostrup, Denmark | 1:50 (IHC, IF) |
anti-CD31 | Rabbit | ab28364 | Abcam, Cambridge, UK | 1:50 (IF) |
anti-α-SMA | Rabbit | ab5694 | Abcam, Cambridge, UK | 1:100 (IF) |
anti-c-kit/CD117 | Rabbit | A4502 | Dako, Glostrup, Denmark | 1:200 (IF) |
anti-PDGFRα | Goat | AF-307-NA | R&D Systems, Minneapolis, MN, USA | 1:100 (IF) |