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. 2019 Apr 10;9:5847. doi: 10.1038/s41598-019-42396-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Growth kinetics of C. trachomatis in primary human Sertoli cells. Infectivity profile (A), chromosomal replication (B), quantification of the inclusion size (C) and fluorescence micrographs (D). Infected primary human Sertoli cells were removed for analysis at 4 hourly intervals and then titrated to assess the quantity of IFUs and the relative number of chlamydial genomes by qPCR. Values are expressed as means ± SD of four replicates from two independent experiments. Chlamydial inclusions were visualized by indirect immunofluorescence with species-specific anti-MOMP monoclonal antibodies (Mab6ciii). Representative images of ten chlamydial inclusion per time-point are shown. Mean and standard deviation of inclusion size, expressed as square pixels, were measured from fluorescence micrographs using ImageJ software.