Fig. 2.

MYC and RUNX2 collaborate to promote the formation of BPDCN cells. a Expression levels of MYC mRNA in CAL-1 cells transduced with two distinct MYC-directed shRNA vectors and a control luciferase-directed shRNA vector. b Expression levels of the MYC protein in MYC KD CAL-1 cells. Levels of β-Actin were used as loading controls. c Impaired colony formation capacities in MYC KD CAL-1 cells from those in control cells (n = 3). d Expression levels of RUNX2 mRNA in CAL-1 cells transduced with two distinct RUNX2-directed shRNA vectors and a control luciferase-directed shRNA vector. e Expression levels of the RUNX2 protein in RUNX2 KD CAL-1 cells. Levels of β-Actin were used as loading controls. f Impaired colony formation capacities in RUNX2 KD CAL-1 cells from those in control cells (n = 3). g Decreased cell growth in RUNX2 KD CAL-1 cells (squares and triangles) under in vitro liquid culture conditions from that in control CAL-1 cells (circles) (n = 3). h Reduced proliferative capacities in RUNX2 KD CAL-1 cells assessed by BrdU incorporation (n = 3). i Enhanced apoptosis in RUNX2 KD CAL-1 cells over that in control CAL-1 cells (n = 3). j Reduced colony formation capacity in MYC and RUNX2 double KD CAL-1 cells from those in single KD cells (n = 3). a, c, d, f, g–j Bars show the mean±SD, *p < 0.05, **p < 0.01, and ***p < 0.001 by the Student’s t-test. Data are representative of 2–3 independent experiments