Fig. 6.

Transduction of MYC and RUNX2 promote the production of pDCs with the serial replating property. a Experimental scheme utilizing MYC and RUNX2 vectors and Tet2/p53 DKO mice for in vitro and in vivo assays. b Representative flow cytometric profiles of the CD45⁺CD11b⁻-gated CD11c⁺B220⁺ cells. c Absolute numbers of CD11b⁻CD11c⁺B220⁺ pDCs among 1000 LSK cells-derived CD45⁺ hematopoietic cells under liquid culture conditions (n = 6). d Representative colony images (top columns) and GFP (bottom columns) of WT and DKO cells expressing MYC plus RUNX2 in the first plating. e Increased colony-forming properties of DKO cells expressing MYC and DKO cells expressing MYC plus RUNX2 (n = 3). f Enhanced serial re-plating properties of DKO cells expressing MYC plus RUNX2 (black bars) from those of WT cells (opened bars), WT cells expressing MYC plus RUNX2 (gray bars) and DKO (dark gray bars) (n = 3). c, e, f Bars show the mean±SD, *p < 0.05 and **p < 0.01 by the Student’s t-test. N.D. not detected. Data are representative of 2–4 independent experiments