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. 2019 Apr 10;9:5865. doi: 10.1038/s41598-019-41883-x

Table 3.

Presence and absence of accessory virulence genes in V. cholerae O139 genome assemblies.

Accessory virulence gene (N16961 locus ID or accession number) Present in 48853_F01 Present in 48853_G01 Present in 48853_H01 Present in 48853_A02
ToxR (VC_0984) Yes Yes Yes Yes
Zona occludens toxin, Zot (VC_1458) No Yes Yes Yes
Accessory cholera enterotoxin, Ace (VC_1459) No Yes Yes Yes
Haemolysin, hlyA (VC_A0219) Yes Yes Yes Yes
Mannose-sensitive haemagglutinin, MSHA (VC_0398..VC_0414) Yes Yes Yes Yes
MARTX toxin, rtxA (VC_1451) Yes Yes Yes Yes
MARTX toxin accessory gene, rtxC (VC_1450) Yes Yes Yes Yes
HA/protease, hapA (VC_A0865) Yes Yes Yes Yes
Heat-stable enterotoxin NAG-ST (Accession # M85198.1) No No No No
Type III secretion system from V. cholerae AM_19226 (typically present in lieu of VPI-2; accession # AATY01000000) No No No No

Gene presence and absence was determined using ACT67 to visualise BLASTn synteny plots, and using tBLASTx to scan assemblies using the NAG-ST nucleotide sequence as a query.