Fig. 7.

Cellular paroxysm biological exploration. Exploration of cytoskeletal (a) and membrane (b) disruption during cellular paroxysm using Alexa Fluor 488 Phalloidin and Annexin V-FITC, respectively. (row 1) Representative Σ_s maps of cells before UV irradiation, scaled from 0.00012–0.00079. (row 2) Multiple m_f maps processed and combined to show which cells experienced the cellular paroxysm event throughout the experiment. (row 3) Representative fluorescence image of Fluor 488 Phalloidin (a) and Annexin V-FITC (b). (row 4) a Box plot showing distribution of the normalized phalloidin intensity and b bar graph counting cells that are grouped based on their Annexin V state [positive (Ann+) or negative (Ann−)] and whether they experienced the cellular paroxysm (CP) or not (NCP). The cellular paroxysm is correlated to both cytoskeletal and membrane disruption. The box within the plot represents the first quartile, median (center line), and third quartile; the whiskers extend to the minimum and maximum values excluding any outliers (1.5x the interquartile range above the third quartile or below the first quartile; outliers not shown). Scale bar is 11 µm. Reported p-values are from two-tailed, heteroscedastic Student’s t-tests